Desarrollar protocolos de citometría de flujo multiparametrica como técnica que ácido lactobiónico en un sistema de cultivo en discontinuo con alimentación. de flujo continuo, y se analizaron sus efluentes, según los tiempos de retención hidráulica biodigestores se observó variación en la persistencia de agentes patógenos, indicando que funcionan secuenciales discontinuos (12). Caceres E.R. and Chiliquinga, B. () “Digestores discontinuos o por lotes “*Biodigestores anaeróbicos de flujo ascendente para la depuración de líquido.
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Quiero y debo agradecer en primer lugar dee Prof. Tengo muy claro de que si aspiro a ser doctor es gracias al Dr. Manuel Rendueles, quien ha sabido guiarme en el trabajo diario. Quisiera expresar mi enorme gratitud a las Dras. Quisiera asimismo agradecer al Prof.
Clark llamada Biotechnology and Bioengineering. Igualmente quiero expresar mi gratitud a la Dra. Ciertamente, ha sido un verdadero placer contar con la biodigestoes de verdaderas profesionales. Igualmente tengo que agradecer a la Dra. En primer lugar agradecer a la Dra.
Federico Gularte un verdadero gentleman por sus sabios y acertados consejos. Ha sido un verdadero placer trabajar contigo, Ire!. Vales millones de euros Jorge!.
Han sabido comprenderme y aguantarme en los malos tiempos, que ciertamente han sido bastantes.
Han tenido una infinita paciencia conmigo porque la verdad es que cuando las cosas no salen en el laboratorio, uno se desespera y a veces se convierte en inaguantable. A mi hermano le agradezco que me haya echado biodigesfores mano en el tramo final por la gran portada que me ha fabricado.
Han sido un apoyo moral fundamental para culminar este trabajo. Likewise, sustainability has been one of the challenges facing the biotechnology industry, which has been focused on both the development of efficient strategies and the biotechnological use of industrial wastes or byproducts.
Within this context, this thesis has addressed the biotechnological production of two commercial organic acids such as lactic and lactobionic acid from two food wastes, cheese whey and yoghurt whey. Firstly, it was studied the lactic acid production by Lactobacillus casei from fermentable sugars, which are available in the yoghurt whey removed from yoghurt derivates out of shelf life.
Thus, the fermentative capacity of L. In order to gain insight into the fermentative behaviour, multiparameter flow cytometry has been used as tool for the evaluation of cell functionality during the bioprocess. In fact, the application of this technique has enabled to know the cellular physiological status and system robustness at the population level.
Likewise, the bio-production lactobionic acid from whey by Pseudomonas taetrolens was studied under different bioprocessing conditions. Fflujo acid has recently emerged into the market with a plethora of applications in areas such as food and biomedicine due to its excellent properties.
Obtención de ácidos orgánicos por fermentación de subproductos lácteos
To date, its industrial production is restricted to chemical catalysis, resulting in an inefficient and unsustainable methodology owing to costly catalysts as well as the formation of side-reaction products. The biotechnological production may overcome these drawbacks as the most appropriate alternative in order to meet the increasing demand of lactobionic acid. The bioprocess developed in the present study has been carried out at different scales in order to decipher the rate-limiting steps, optimizing the system based on appropriate operational considerations both for physiology and yield of P.
Monitorization of the physiological heterogeneity through multiparameter flow cytometry tlujo thus provided precise knowledge about the functional behaviour of P. The present study has consequently added further knowledge on how different bioprocessing factors such as the operational ph, dissolved oxygen availability or biodigestorez age may play a decisive role in the. Esta excesiva oferta ha. Conocer el comportamiento fermentativo de L. Igualmente se presentan los objetivos que se han pretendido alcanzar.
De hecho, en el mercado se encuentran productos afianzados comercialmente como la lisina, el glutamato riscontinuo el bioetanol. Datos discomtinuo de Soetaert y Vandamme De hecho, se han conseguido cepas recombinantes de E.
Obtención de ácidos orgánicos por fermentación de subproductos lácteos – PDF
Bio-production of lactobionic acid: Lactobionic acid has appeared on the commercial scene as a versatile polyhydroxy acid with numerous promising applications in the food, medicine, pharmaceutical, cosmetics and chemical industries. This high value-added bio-product has recently received growing attention as a bioactive compound, providing an excellent chemical platform for the synthesis of novel potentially biocompatible and biodegradable drug delivery vehicles.
Recent advances in tissue engineering and nanomedicine have also underlined the increased importance of this organic acid as a key biofunctionalization agent. The growing commercial relevance of lactobionic acid has therefore prompted the development of novel systems for its biotechnological production that are both sustainable and disccontinuo.
The present review explores recent advances and studies related to lactobionic acid bio-production, whether through microbial or enzymatic approaches, highlighting the key bioprocessing conditions for enhanced bio-production. Detailed overviews of the current microbial cell factories as well as downstream processing methodologies for lactobionic acid production are also presented. Furthermore, the potential prospects and current applications of this polyhydroxy acid are also discussed, with an emphasis on the role of lactobionic acid as a key platform in the development of novel biodigestorea, biomaterials, nanoparticles and biopolymer systems.
two-phase anaerobic biodigestor: Topics by
Introduction The production of bulk organic acid chemicals by microbial fermentation has undoubtedly undergone continuing growth over the last decade, progressively expanding its market niche and portfolio Jang et al. In fact, bacteria as bioproduction platforms have become a reliable, cost-competitive, feasible alternative for large-scale industrial production of many bulk and specialty organic acids Demain, Beyond traditional organic acids, the market has also shown substantial interest in novel carboxylic acids like lactobionic acid due to its unique physicochemical properties Fig.
As a result, novel lactose derivatives such as lactitol, lactulose and LBA have recently come onto the commercial market with considerable industrial applications Playne and Crittenden, ; Seki and Saito, Current trends in the organic acids market. The market is moving from commodity bulk carboxylic acids towards value-added organic acids which display outstanding applications. LBA currently belongs to this latter segment. In recent years, LBA has also received growing attention as a bioactive molecule since it provides an excellent platform for the synthesis of biocompatible and biodegradable drug.
In this respect, LBA will clearly play a major strategic role in the treatment of hepatic disorders through nanomedicine, with a potential near-term impact.
Its prospect as a key biomolecule in the field of nanotechnology is thus of outstanding significance. In view of this commercial relevance, both the development and implementation of feasible LBA production systems emerge as crucial key challenges to meet market demands. To date, LBA is manufactured by chemical synthesis in an energyintensive process which requires the use of costly metal catalysts Kuusisto et al.
However, this expensive methodology may also involve the generation of undesirable side-reaction products Chia et al. Although this polyhydroxy acid has been available since the late s Stodola and Lockwood, ; Stodola and Jackson,its production by biotechnological means has not been developed so intensively up to now in comparison with other organic acids such as lactic, succinic or citric acid Papagianni, Nevertheless, bio-production of LBA has emerged as both a promising and feasible approach to meet the growing demand for this bio-product.
Furthermore, environmentally-friendly and cost-effective LBA bioproduction can be accomplished by employing cheese whey as an inexpensive feedstock Alonso et al. Despite being a traditional natural source for whey protein isolate Smithers, and lactose Schaafsma,cheese whey upgrading and treatment remain as two of the major challenges facing the dairy industry.
Within this context, the present review explores recent advances in LBA bio-production, either through enzymatic or microbial biosynthesis, as well as the current novel trends addressing the application of LBA in the marketplace, with particular emphasis on those emerging areas such as nanomedicine and tissue engineering. A detailed overview of current microbial cell factories, further downstream processing methodologies for LBA production and prospects are also provided.
Properties and current industrial status of lactobionic acid. The structure and physicochemical properties of LBA confer on it a plethora of current and potential commercial applications, as shown in Figure 2. This organic acid exhibits a large number of newly discovered biological activities and great therapeutic potential due to its excellent biocompatibility, biodegradability and nontoxicity, as well as its chelating, amphiphilic and antioxidant properties.
LBA belongs to the aldobionic family of acids, which. Chemically, it comprises one galactose molecule attached to biodigeatores molecule of gluconic acid via an biodigestpres linkage its molecular structure is shown in Table 1.
In particular, the presence of multifunctional groups 8 hydroxyl groups converts LBA molecule into a metal ion chelator which can sequester cations such as calcium Abbadi et al. This carboxylic acid presents a molecular weight of Table 1 lists the main physicochemical properties of LBA; its high water solubility being especially noteworthy.
The calcium salt of LBA in fact shows 40, times higher water solubility than calcium carbonate and 10 times that of calcium lactate Nakano et al. Overview of LBA applications.
LBA was reported and synthesized for the first time by Fischer and Meyer as the oxidation product of the lactose free aldehyde group after a chemical oxidation with Bromine.
In the industrial manufacturing context, LBA is currently produced by chemical synthesis from refined lactose in an energy-intensive process Kuusisto et al. This manufacturing process also involves the use of harmful and costly catalysts, as well as the generation of undesirable side-reaction products Murzina et al. Aside from bio-production methods, catalytic wet oxidation and electrochemical catalysis of lactose have also been proposed as methodologies to obtain LBA.
Likewise, increasing environmental requirements disccontinuo undoubtedly drive LBA industrial production towards more sustainable production systems that, to date, otherwise not complied with chemical catalytic oxidation or enzymatic methods. Physicochemical properties of LBA. Although no official data are available on its annual world production Playne and Disontinuo,LBA has burst onto the commercial scene as a prominent emerging substance with a significant improved commercial rate in recent years Affertsholt, In view of LBA market forecasts, Solvay in fact opened a plant in Germany in the late s with the aim of achieving an annual production of tons Gerling, Targeted delivery of therapeutic agents has thus emerged as a promising approach in medicine not only due to its increased therapeutic efficacy, but also to its lesser side effects.
biodigestor de flujo continuo pdf
In fact, the lack of effective and practical pharmacological approaches for targeting liver cells has become the driving force behind the development of effective drug-delivery systems employing LBA as a ligand. In this context, LBA offers unique properties such as biocompatibility, biodegradability, ion-chelating ability and selfassembly, in addition to their synergistic combination, which confer on LBA a plethora of applications as a vehicle for the innovative treatment of many potential diseases.
Owing to these unique properties, LBA provides an excellent platform for the synthesis of potentially biocompatible and targetable drug delivery vehicles, from DNA to bioactive molecules. Table 2 lists recent research studies that have focused on the development and assessment of drug-delivery systems containing LBA as a ligand.
The current growing prevalence of hepatic disorders has become the driving force behind the development of novel therapeutic treatments based on hepatic-targeted drug delivery systems Chen et al. Targeting hepatoma cells constitutes a promising approach for clinical chemotherapy of liver cancer which is currently a subject of intensive research efforts Chen et al.
LBA-based drug delivery systems can successfully target hepatocytes due to the presence of asialoglycoprotein receptors ASGPR located on their surface, since LBA works as a ligand of these receptors Kim et al.
These receptors represent the preferential targets for potential hepatic interventions, as the therapeutic effect of any drug is often limited by the difficulty of accessing specific sites. This receptor additionally provides a membrane-bound active site for cell-to-cell interactions Stockert, The functionalization of LBA may thus achieve enhanced binding to the cell surface on hepatocytes Park et al. Furthermore, successful receptor-mediated cell transfection in targeted specific tissues such.
Comparison of research studies using LBA as the target ligand for drug delivery purposes. Hepatocytes Zheng et al.
Hepatocytes Yang et al. Hepatocytes Zhang et al. Spermine HepG2 Alex et al. Poly-L-lysine HepG2 Choi et al.